日韩一区二区三区在,亚洲成a人v天堂网,免费收看人成电影,女人15毛片A级15女人水真多,天天操天天爱,欧美性爱A黄毛性胶片

服務(wù)熱線：
19050699345
相關(guān)技術(shù)文章/ ARTICLES
產(chǎn)品展示

您現(xiàn)在的位置：首頁(yè)  >  產(chǎn)品展示  >    >  常規(guī)ELISA試劑盒  >  D1045 5-Hydroxymethyl dCTP

D1045 5-Hydroxymethyl dCTP

  • 產(chǎn)品型號(hào)：
  • 產(chǎn)品時(shí)間：2024-08-09
  • 簡(jiǎn)要描述：D1045 5-Hydroxymethyl dCTP 上?；鄯f生物優(yōu)勢(shì)供應(yīng)，*，！
    上海慧穎生物，值得您信賴的選擇?。?/li>
  • 產(chǎn)品簡(jiǎn)介

D1045 5-Hydroxymethyl dCTP   

品牌：ZYMO Research

代理商：慧穎生物

庫(kù)存：貨期產(chǎn)品

D1045 5-Hydroxymethyl dCTP   

Ready to use 5-Hydroxymethylcytosine mix (dATP, dTTP, dGTP, d5hmCTP) and 5-Methylcytosine dNTP mix (dATP, dTTP, dGTP, d5mCTP) is of ultra high purity; >99% trisphosphate by HPLC.

Readily incorporated into PCR amplicons with ZymoTaq™ or other DNA polymerases.

Free of endo-, exodeoxyribonuclease, ribonuclease, phosphatase and nicking activities.

About Hydroxy/Methylated Nucleotides

5-(Hydroxy)methylcytosine dNTP Mix and 5-(Hydroxy)methyl dCTP from Zymo Research are of ultra high purity and can be used to generate DNA by PCR using ZymoTaq™ or other DNA polymerases.

 For 5-hmCFor 5-mC
ReagentVolumeFinal ConcentrationVolumeFinal Concentration
2X Reaction Buffer25 µl1X25 µl1X
dNTP Mix0.5 µl0.25 mM each dNTP0.5 µl0.25 mM each dNTP
Forward Primer (10 µM)Variable0.3 to 1 µMVariable0.3 to 1 µM
Reverse Primer (10 µM)Variable0.3 to 1 µMVariable0.3 to 1 µM
Template DNAVariable< 200 ng/50 µlVariable< 200 ng/50 µl
ZymoTaq™ DNA Polymerase (5 U/µl)1 µl5 U/50 µl0.4 µl<2 U/50 µl
ddH20to 50 µl-to 50 µl-
Total Volume50 µl 50 µl 
 
Note: The final concentration of MgCl2 in the reaction (above) is 1.75 mM. If required, scale reaction reagent volumes accordingly to optimize the MgCl2, primer, and/or template concentrations.
Specifications
Concentration5-Hydroxymethylcytosine dNTP Mix: 2.5 µmol/250 µl as a [10 mM] solution. Contains [2.5 mM] of each dNTP (dATP, dTTP, dGTP and d5hmCTP). 
5-Hydroxymethyl dCTP: 10µmol/100 µl as a [100 mM] solution.
5-Methylcytosine dNTP Mix: 2.5 µmol/250 µl as a [10 mM] solution. Contains [2.5 mM] of each dNTP (dATP, dTTP, dGTP and d5mCTP).
5-Methyl dCTP: 1µmol/100 µl as a [10 mM] solution.
ApplicationsPCR amplicons containing 5-(hydroxy)methylcytosine are useful in a variety of applications such as: 
  • The study of 5-(hydroxy)methylcytosine binding/interacting protein
  • Define methods that distinguish between 5-methylcytosine and 5-hydroxymethylcytosine.
Storage5-(Hydroxy)methylcytosine dNTP Mix and 5-(Hydroxy)methyl dCTP are guaranteed for 12 months at -20°C. Avoid multiple freeze/thaw cycles. For long-term storage, aliquoting is recommended with storage at -80°C.

留言框

  • 產(chǎn)品：

  • 您的單位：

  • 您的姓名：

  • 聯(lián)系電話：

  • 常用郵箱：

  • 省份：

  • 詳細(xì)地址：

  • 補(bǔ)充說(shuō)明：

  • 驗(yàn)證碼：

    請(qǐng)輸入計(jì)算結(jié)果（填寫阿拉伯?dāng)?shù)字），如：三加四=7

上海慧穎生物科技有限公司 版權(quán)所有    備案號(hào)：滬ICP備12016933號(hào)-2

技術(shù)支持：化工儀器網(wǎng)    管理登陸    網(wǎng)站地圖

聯(lián)系電話：
15821734033

微信服務(wù)號(hào)